Genome sequencing

Research

Genome sequencing

Genome sequencing is a determination of the complete nucleotide sequence of an organism’s genome. Our group has sequenced the genome of Arthrospira platensis C1 using a hybrid method in which the 454 Life Sciences technology on the Genome Sequencer (GS) FLX System and BigDye Terminator v3.1 Cycle sequencing were employed. Pyrosequencing reads were assembled using the Newbler de novo sequence assembly software version 2.0.0 (Roche). The Phred/Phrap/Consed software package was used for sequence assembly and quality assessment in the finishing process. The remaining gaps between contigs were closed by custom primer walk or PCR amplification and then editing in Consed. The final assembly contains 739,684 reads from pyrosequencing and 45,959 reads from Sanger sequencing, resulting in 28× coverage of the genome. Employing A. maxima CS-328 contigs as a reference, the A. platensis C1 circular genome of 6.08 Mb total size with 1 scaffold and 63 gaps has been constructed. The genome sequencing result and its corresponding annotation data are summarized in Table 1.

Table 1. Arthrospira platensis C1 Genome statistics

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